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Figure 2. mRNA expression levels of <t>VEGF</t> and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.
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Figure 2. mRNA expression levels of <t>VEGF</t> and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.
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Figure 2. mRNA expression levels of <t>VEGF</t> and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.
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Figure 2. mRNA expression levels of <t>VEGF</t> and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.
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<t>VEGFC</t> and VEGFR2 protein levels in conditioned media of stable cell lines indicated <t>by</t> <t>ELISA.</t> (A) VEGFC; (B) VEGFR2. VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay.
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(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular <t>endothelial</t> growth factor <t>(VEGF)</t> levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).
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(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular <t>endothelial</t> growth factor <t>(VEGF)</t> levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).
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(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular <t>endothelial</t> growth factor <t>(VEGF)</t> levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).
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(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular <t>endothelial</t> growth factor <t>(VEGF)</t> levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).
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(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular <t>endothelial</t> growth factor <t>(VEGF)</t> levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).
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(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular <t>endothelial</t> growth factor <t>(VEGF)</t> levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).
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Image Search Results


Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 2. mRNA expression levels of VEGF and MMP‑13 in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Relative mRNA expression levels of VEGF in the medial articular cartilage. (B) Relative mRNA expression levels of MMP‑13 in the medial articular cartilage. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; MMP‑13, matrix metalloproteinase‑13; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation

Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 3. Immunohistochemical analysis of VEGF expression in the knee articular cartilage of mice among the Sham, Dmm and Dmm+Th groups (n=4 in each group). (A) Immunohistochemistry staining of VEGF in the articular cartilage of the medial tibial plateau (magnification, x400, scale bar=100 µm). (B) Quantification of VEGF positive cells, based on the results of immunohistochemistry staining. The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Immunohistochemical staining, Expressing, Immunohistochemistry, Staining, Standard Deviation

Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Journal: Molecular medicine reports

Article Title: Intraperitoneal injection of thalidomide alleviates early osteoarthritis development by suppressing vascular endothelial growth factor expression in mice.

doi: 10.3892/mmr.2018.8980

Figure Lengend Snippet: Figure 5. ELISA analysis of serum VEGF concentration of mice among the Sham, Dmm and Dmm+Th groups (n=8 in each group). The values are presented as the mean ± standard deviation. *P<0.05 compared with the Sham group; #P<0.05 compared with the Dmm group. Dmm, destabilization of the medial meniscus; Th, thalidomide; VEGF, vascular endothelial growth factor.

Article Snippet: An ELISA kit of VEGF (E-EL-M1292c) was purchased from Elabscience Biotechnology Co., Ltd., Wuhan, China.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Standard Deviation

VEGFC and VEGFR2 protein levels in conditioned media of stable cell lines indicated by ELISA. (A) VEGFC; (B) VEGFR2. VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay.

Journal: Chinese Journal of Cancer Research

Article Title: Tim-3 promotes cell aggressiveness and paclitaxel resistance through NF-κB/STAT3 signalling pathway in breast cancer cells

doi: 10.21147/j.issn.1000-9604.2020.05.02

Figure Lengend Snippet: VEGFC and VEGFR2 protein levels in conditioned media of stable cell lines indicated by ELISA. (A) VEGFC; (B) VEGFR2. VEGF, vascular endothelial growth factor; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: ELISA was performed using the human VEGFC (cat. no. E-EL-H1600; Elabscience) and vascular endothelial growth factor receptor 2 (VEGFR2) ELISA kit (cat. no. E-EL-H1603; Elabscience), according to the manufacturer’s protocol.

Techniques: Stable Transfection, Enzyme-linked Immunosorbent Assay

(A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular endothelial growth factor (VEGF) levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).

Journal: Mediators of Inflammation

Article Title: Calcitriol Administration Attenuates Acute Lung Injury by Improving Angiopoietin/Tie2 Dysregulation and Alveolar-Capillary Barrier Integrity in a Mouse Model of Ovariectomy Complicated With Sepsis

doi: 10.1155/mi/4374164

Figure Lengend Snippet: (A) Angiopoietin (ang)/tyrosine kinase with immunoglobulin-like and epidermal growth factor-like domain 2 (Tie2) pathway-associated proteins and (B) vascular endothelial growth factor (VEGF) levels in lung tissues. Calcitriol treatment showed higher Ang1, Tie2, and VEGF levels and an Ang1/Ang2 ratio in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) ( n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP ( n = 16), sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP ( n = 16), sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72). Values are expressed as the mean ± SEM. Comparisons among groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. # Significantly differs from the OVSS groups on 24 and 72 h. ⁣ ∗ Significantly differs from the OVSS group at the same time point ( p < 0.05).

Article Snippet: Ang1, Ang2, vascular endothelial growth factor (VEGF), and Tie2 were analyzed using enzyme-linked immunosorbent assay (ELISA) kits (Ang1, OKEH00011; Ang2, OKEH05460; VEGF, OKRC01327; Tie2, OKEH00011, AVIVA Systems Biology, San Diego, CA, USA).

Techniques: Ligation, Saline, Injection